![]() ![]() In both small and large caprine islets ( 150 μm in diameter, respectively), the majority of β-cells were positioned at the core and α-cells were arranged at the mantle, while some single α-cells were also observed in the islet center. Such cytoarchitecture was observed in all examined caprine pancreatic islets and was also reported for the islets of other ruminants. In caprine islets, α- and δ-cells remarkably were intermingled with β-cells in the mantle. Histological sections of caprine pancreatic islets showed that α-cells were segregated at the periphery of β-cells. Batches of islet were selected for immunostaining and study through confocal microscopy and flow cytometry. MethodologyĪfter collection and purification of caprine islets with Euro-Ficoll density gradients, islets were considered for viability and functionality procedures with DTZ (dithizone) staining and GSIST (glucose-stimulated insulin secretion test) subsequently. This investigation aimed to characterize caprine islets in regard to better understanding of caprine islet structure and compare with previously reported species, by conducting a detailed analysis of islet architecture and composition using confocal microscopy and immunofluorescence staining for pancreatic islet hormones. Previous studies have shown that cell arrangement in the pancreatic islets of ruminants differs from that of other mammals however, caprine islet cytoarchitecture has not yet been comprehensively described. The scarcity of pancreatic islet donors for patients with diabetes has caused a considerable interest in the field of islet xenotransplantation. Every islet cell type is distinguished by its specific secretory granule pattern and hormone content, endocrine and cell signaling mechanisms, and neuronal interactions. A finely balanced combination of endocrine cells in the islets regulates intraportal vein secretions and plasma nutrient levels. Pancreatic islets are composed of different hormone-secreting cell types.
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